microscope inverted stand Search Results


ix83 inverted microscope stand  (Yokogawa Electric)
99
Yokogawa Electric ix83 inverted microscope stand
Ix83 Inverted Microscope Stand, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/CSU-W1/bio_rxiv__2021__11__11__468235-273-19-23
Average 99 stars, based on 1 article reviews
ix83 inverted microscope stand - by Bioz Stars, 2026-11
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spinning disk confocal microscope  (Yokogawa Electric)
99
Yokogawa Electric spinning disk confocal microscope
Spinning Disk Confocal Microscope, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/CSU-X1/bio_rxiv__2024__10__18__618370-222-13-17
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spinning disk confocal microscope - by Bioz Stars, 2026-11
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inverted microscope stand  (Nikon)
99
Nikon inverted microscope stand
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Inverted Microscope Stand, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/Objectives/pmc05912900-238-65-70
Average 99 stars, based on 1 article reviews
inverted microscope stand - by Bioz Stars, 2026-11
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eclipse ti inverted microscope stand  (Nikon)
99
Nikon eclipse ti inverted microscope stand
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Eclipse Ti Inverted Microscope Stand, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/Inverted+Microscopes/bio_rxiv__2021__06__03__446912-251-15-14
Average 99 stars, based on 1 article reviews
eclipse ti inverted microscope stand - by Bioz Stars, 2026-11
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nikon ti2 inverted microscope  (Nikon)
94
Nikon nikon ti2 inverted microscope
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Nikon Ti2 Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/Stands/pmc12516322-210-6-6
Average 94 stars, based on 1 article reviews
nikon ti2 inverted microscope - by Bioz Stars, 2026-11
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dmi8 inverted microscope stand  (Danaher Inc)
99
Danaher Inc dmi8 inverted microscope stand
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Dmi8 Inverted Microscope Stand, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/DMi8+S+Inverted+Microscope+Solution/pm35977489-220-16-15
Average 99 stars, based on 1 article reviews
dmi8 inverted microscope stand - by Bioz Stars, 2026-11
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axio observer 7 inverse microscope  (Carl Zeiss)
98
Carl Zeiss axio observer 7 inverse microscope
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Axio Observer 7 Inverse Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/Microscope+stand+Axio+Observer+7/pmc09459136-183-21-26
Average 98 stars, based on 1 article reviews
axio observer 7 inverse microscope - by Bioz Stars, 2026-11
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inverted microscope stand  (Carl Zeiss)
99
Carl Zeiss inverted microscope stand
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Inverted Microscope Stand, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/Inverted+microscope+Axio+Observer+3/pmc07319019-293-22-28
Average 99 stars, based on 1 article reviews
inverted microscope stand - by Bioz Stars, 2026-11
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stereomicroscope  (Carl Zeiss)
97
Carl Zeiss stereomicroscope
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Stereomicroscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/Stereomicroscope+SteREO+Discovery%2EV8+with+stand+300/pmc03696330-117-29-32
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stereomicroscope - by Bioz Stars, 2026-11
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axio vert a1 invered microscope  (Carl Zeiss)
97
Carl Zeiss axio vert a1 invered microscope
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Axio Vert A1 Invered Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/Microscope+stand+Axio+Vert%2EA1/pmc09285673-290-2-7
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axio vert a1 invered microscope - by Bioz Stars, 2026-11
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axio observer z1 7  (Carl Zeiss)
97
Carl Zeiss axio observer z1 7
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Axio Observer Z1 7, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/Shutter+FL%2C+internal+for+stands+Axio+Observer+3+%2F+5+%2F+7+%2F+D1+%2F+Z1/pm38897036-100-8-10
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axio observer z1 7 - by Bioz Stars, 2026-11
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stand ix83  (Olympus)
99
Olympus stand ix83
cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. <t>Microscope</t> icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .
Stand Ix83, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+inverted+stand/IX83+Inverted+Microscope+Currently+integrated+into+IXplore+Systems/pmc08469251-131-16-18
Average 99 stars, based on 1 article reviews
stand ix83 - by Bioz Stars, 2026-11
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Image Search Results


cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. Microscope icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .

Journal: Current Biology

Article Title: A cAMP/PKA/Kinesin-1 Axis Promotes the Axonal Transport of Mitochondria in Aging Drosophila Neurons

doi: 10.1016/j.cub.2018.02.048

Figure Lengend Snippet: cAMP Boosts Mitochondrial Transport in Wing Neurons of Aged Flies (A) Cartoon of the Drosophila wing. Green: sensory neurons used in this study. Magenta box: region imaged for transport studies, which contains bundled axons from several neurons. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of 30-day-old flies (“aged” flies) expressing UAS-luciferase (luc) RNAi (control) or UAS-dnc RNAi . Bottom: traces of transported mitochondria in corresponding movies. (C) Number of transported and total mitochondria per 50 μm of axonal tract in wings of aged flies. In these and other experiments, each wing was filmed for 3 min. (D) Overview of 8-Br-cAMP feeding experiments. Green square: period of feeding. Microscope icon: visualization of mitochondrial transport. (E) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of flies 32 days after eclosion following 4 days of feeding with vehicle or 8-Br-cAMP. Bottom: traces of transported mitochondria in corresponding movies. (F and G) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (F) and young (G) flies after 4 days of feeding with vehicle or 8-Br-cAMP. (H) Percentage of axonal segments of wing neurons in 5-week-old flies that contained focal accumulation of GFP after feeding throughout adulthood with vehicle or 8-Br-cAMP. dpr-Gal4 is expressed in the chemosensory neurons of the wing. Statistical significance was evaluated with a Mann-Whitney U test (C, F, and G) or two-tailed Student’s t test (H). ∗ p < 0.05. Magenta circles are values for individual wings, except in (H), where they are values from individual Z projections from 13 wings per genotype; error bars are SEM. Scale bars, 5 μm. See also and , and , and .

Article Snippet: Two spinning disk systems were used during the study, with the same system used for an entire experimental series: (1) PerkinElmer Ultraview ERS with a CSU21 scanning head (Yokogawa) and an inverted microscope stand (IX71 (Olympus)) equipped with a 60x PlanApo oil-immersion objective (1.4 NA) and a CCD camera (Orca ER (Hamamatsu)); (2) Nikon spinning disk system with a CSU-X1 scanning head (Yokogawa) and an inverted microscope stand (Eclipse Ti-E (Nikon)) equipped with a 60 x CFI Apo oil-immersion objective (1.4 NA) and an EM-CCD camera (Du 897 iXon Ultra (Andor)).

Techniques: Labeling, Expressing, Luciferase, Control, Microscopy, MANN-WHITNEY, Two Tailed Test

PKA-Induced Upregulation of Mitochondrial Transport in Wing Neurons of Aged Flies (A) Overview of the procedure for heat-shock (hs)-mediated induction of PKA ∗ . Green square: period of heat shock. Microscope icon: visualization of mitochondrial transport. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of aged control and hs-PKA ∗ flies that have been subjected to heat shock. Bottom: traces of transported mitochondria in corresponding movies. (C and D) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (C) and young (D) control or hs-PKA ∗ flies subjected to heat shock. (E) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons of 2-day-old flies with expression of UAS-luciferase RNAi or two independent UAS-Pka-C1 RNAi constructs. appl-Gal4 marks both the mechanosensory and chemosensory neurons in the wing nerve. Statistical significance was evaluated with a Mann-Whitney U test (C and D) or a one-way ANOVA with Dunnett’s multiple comparison (E). ∗ p < 0.05, ∗∗ p < 0.01. Magenta circles are values for individual wings; error bars are SEM. Scale bar, 5 μm. See also and , , and .

Journal: Current Biology

Article Title: A cAMP/PKA/Kinesin-1 Axis Promotes the Axonal Transport of Mitochondria in Aging Drosophila Neurons

doi: 10.1016/j.cub.2018.02.048

Figure Lengend Snippet: PKA-Induced Upregulation of Mitochondrial Transport in Wing Neurons of Aged Flies (A) Overview of the procedure for heat-shock (hs)-mediated induction of PKA ∗ . Green square: period of heat shock. Microscope icon: visualization of mitochondrial transport. (B) Top: stills from movies of GFP-labeled mitochondria in wing neuron axons of aged control and hs-PKA ∗ flies that have been subjected to heat shock. Bottom: traces of transported mitochondria in corresponding movies. (C and D) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons in aged (C) and young (D) control or hs-PKA ∗ flies subjected to heat shock. (E) Number of transported and total mitochondria per 50 μm of axonal tract of wing neurons of 2-day-old flies with expression of UAS-luciferase RNAi or two independent UAS-Pka-C1 RNAi constructs. appl-Gal4 marks both the mechanosensory and chemosensory neurons in the wing nerve. Statistical significance was evaluated with a Mann-Whitney U test (C and D) or a one-way ANOVA with Dunnett’s multiple comparison (E). ∗ p < 0.05, ∗∗ p < 0.01. Magenta circles are values for individual wings; error bars are SEM. Scale bar, 5 μm. See also and , , and .

Article Snippet: Two spinning disk systems were used during the study, with the same system used for an entire experimental series: (1) PerkinElmer Ultraview ERS with a CSU21 scanning head (Yokogawa) and an inverted microscope stand (IX71 (Olympus)) equipped with a 60x PlanApo oil-immersion objective (1.4 NA) and a CCD camera (Orca ER (Hamamatsu)); (2) Nikon spinning disk system with a CSU-X1 scanning head (Yokogawa) and an inverted microscope stand (Eclipse Ti-E (Nikon)) equipped with a 60 x CFI Apo oil-immersion objective (1.4 NA) and an EM-CCD camera (Du 897 iXon Ultra (Andor)).

Techniques: Microscopy, Labeling, Control, Expressing, Luciferase, Construct, MANN-WHITNEY, Comparison